Custom Peptide Synthesis services
About peptide solubility
How to dissolve my peptide?
The solubility of peptides varies depending on its amino acid sequence and modifications. When we prepare it, the peptide is generally purified by HPLC using water and acetonitrile gradient. Here are some general tips for dissolving peptides:
sonification will increase the solubility of peptides.
For basic peptides, 10% acetic acid in the solvent will help dissolve peptides.
For acidic peptides, 10% ammonium bicarbonate will help dissolve peptides
For peptides with extremely low solubility in aqueous solutions, organic solvents should be used first (such as DMSO, isopropanol, methanol, acetonitrile). Once peptides are dissolved completely, water may be added slowly until the final concentration has been reached.
Do I need to solubilize my peptide under specific pH?
It will also be stated on your Certificate of Analysis if any specific PH level is requested.
What should I do if my peptide does not dissolve well?
As a general rule, peptides should first be dissolved in distilled, preferably sterile water. If solubility still remains a problem, try the following steps:
Sonication is helpful to dissolve the peptide
Small amounts of dilute(10%) aqueous acetic acid for basic peptides or aqueous ammonia for acidic peptides may help dissolution of these peptides.
It is also recommended that the peptide be dissolved to the highest possible concentration, and then diluted with water or buffer to the working concentration.(Note: The buffer should be added only after the peptide is completely in solution form because salts may cause aggregation).
Why the solubility may vary from one peptide to the other?
Solubility is often one of the major challenges for researchers who work with peptides. Each amino acid exhibits its own physical characteristics. Amino acids such as leucine, isoleucine, and valine are very hydrophobic. Other amino acids like lysine, histidine, and arginine are hydrophilic. Thus, different peptides will have different solubilities, based on the content of the peptides.
How do I solubilize my peptide?
All solubility information is reported on the Certificate of Analysis received with your peptide. Please read the instructions carefully before starting. Solubility may very depending on the nature of the peptide. The most common dissolution process is 1 mg of peptide distilled in 1 ml of sterile water.
About peptide store
Other related question
How should I store my peptide when it is in solution?
If storage of peptides in solution is absolutely unavoidable, use sterile buffers at pH 5-6 and store aliquots at -20℃ to prolong the storage life of peptides in solution.
How long can I store my peptide when it is in solution?
It is not recommended to keep excess peptides in solution. The shelf life of peptides in solution is very limited, especially for sequences containing cysteine, methionine, tryptophan, asparagine, glutamine, and N-terminal glutamic acid. In general, aliquot the necessary amounts of peptide for a few days and relyophilize remaining portions for long term storage if necessary.
Are there any recommended procedures when I am about to start working on my peptide?
When preparing the peptide for use, please observe the following steps to maintain peptide quality: Warm up the peptide to room temperature prior to opening and weighing out portion of the peptide. The recommended time for warm up is 1 hour. In a clean environment weigh out desired quantity of peptide quickly.
Store the remaining peptide in freezer, preferably below -20℃ within an enclosed case with dessicants.
How much does it cost for shipping outside China?
We ship our products all around the world. The oversea shipping charge is generally $40 per shipment for most of countries.
What if some problem happens during the synthesis of my specific peptide?
Each peptide has its specific characteristics. If some problems happen during the synthesis and we cannot deliver your peptide on time, we will inform you as soon as possible.
How do you ship peptides? What data will be provided?
All peptides will be shipped lyophilized in small microcentrifuge tubes (2 ml). Large amount of peptide may be aliquoted into several tubes. For every single peptide, data sheets will be provided that contains the key characteristics, such as amino acid sequence, modification, purity, mass spectrum data and HPLC data.
What is the typical turn-around time for peptide synthesis?
Our typical turn-around time is about 2-3 weeks. The turn-around time may vary depending on the peptide length and difficulty.
