Custom Antibody Production
 
Antibodies (also known as immunoglobulins, abbreviated (Ig) are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. As an antigen is injected into the mammal, it induces the B-lymphocytes to produce IgG immunoglobulins specific for the antigen. The unique part of the antigen recognized by an antibody is called the epitope. These epitopes bind with their antibody in a highly specific interaction. Polyclonal antibodies (or antisera) are antibodies that are obtained from different B cell resources. They are a combination of immunoglobulin molecules secreted against a specific antigen, each identifying a different epitope. Due to polyclonal antibodies recognize multiple epitopes on any one antigen, cause agglutination and precipitation reactions, it is widely used in the field of immunological diagnosis.
 
 
Custom Polyclonal Antibody Services Antibodies (also known as immunoglobulins, abbreviated (Ig) are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. As an antigen is injected into the mammal, it induces the B-lymphocytes to produce IgG immunoglobulins specific for the antigen. The unique part of the antigen recognized by an antibody is called the epitope. These epitopes bind with their antibody in a highly specific interaction. Polyclonal antibodies (or antisera) are antibodies that are obtained from different B cell resources. They are a combination of immunoglobulin molecules secreted against a specific antigen, each identifying a different epitope. Due to polyclonal antibodies recognize multiple epitopes on any one antigen, cause agglutination and precipitation reactions, it is widely used in the field of immunological diagnosis. Polyclonals can help amplify signal from target protein with low expression level, as the target protein will bind more than one antibody molecule on the multiple eptitopes. Due to recognition of multiple epitopes, polyclonals can give better results in IP / ChIP .Polyclonal antibodies are often the preferred choice for detection of denatured proteins.
A reliable immunological assay demands reliable antibodies and antibody services. Our custom antibody production services include recombinant protein expression, peptide design and synthesis, polyclonal antibody production in various animals such as Rabbit、Mouse、Rat、Goat and Chicken, affinity purification, conjugation, fragmentation, and immunoassay development. Phtdpeptides can supplies our customers the most relieable and comprehensive antibody package services to ensure fast, economical and high quality poly-clonal antibody production.
 

Service Code	Service	Brief Description	Time
QY-1001	protein → anti-sera	antigen QC，immunize，ELISA，bleed	60 days
QY-1002	protein → antibody （protinA purification）	antigen QC，immunize，ELISA，Bleed， ProteinA purify	70 days
QY-1003	protein→antibody (antigen affinity purification)	antigen QC，immunize，ELISA，bleed， antigen affinity purify	70 days
QY-1004	peptide → anti-sera	peptide conjugate，immunize，ELISA， Bleed	70 days
QY-1005	peptide → antibody （protinA purification）	peptide conjugate，immunize，ELISA， bleed，proteinA purify	80 days
QY-1006	peptide → antibody （antigen affinity purification）	peptide conjugate，immunize，ELISA， bleed，antigen affinity purify	80 days
QY-1007	peptide sequence  → anti-sera	peptide synthesis,peptide conjugate， immunize，ELISA，bleed	80 days
QY-1008	peptide sequence → antibody （protinA purification）	peptide synthesis,peptide conjugate， immunize，ELISA，bleed，antigen  affinity purify	90 days
QY-1009	peptide sequence → antibody （antigen affinity purification）	peptide synthesis,peptide conjugate， immunize，ELISA，bleed，antigen  affinity purify	90 days
QY-1010	peptide sequence with phosphorylation site  → antibody	peptides synthesis(phosphor peptide  and unmodified peptide )，peptide  conjugate，Immunize，ELISA，Bleed， antigen affinity purify（phosphor  peptide and unmodified peptide）	100 days
QY-1011	peptide sequence with  methyl-specific site → antibody	peptides synthesis(methyl peptide  and unmodified peptide )，peptide conjugate，Immunize，ELISA，Bleed， antigen affinity purification（methyl peptide and unmodified peptide）	100 days
QY-1012	protein sequence  → antibody （WB guaranted in overexpression system）	peptide design,synthesis,and conjugate;Immunize，ELISA，Bleed， antigen affinity purify, WB test(in overexpression system)	100 days
QY-1013	small molecules compound → anti-sera	hapten conjugate，immunize，ELISA， Bleed	75 days
QY-1014	small molecules compound  → antibody （protinA purification）	hapten conjugate，immunize，ELISA， bleed，proteinA purify	85 days
QY-1015	expression Plasmid → anti-sera	protein express and purify，eimmunize ，ELISA，bleed，WB	80 days
QY-1016	expression Plasmid → antibody （protinA purification）	protein express and purify，eimmunize ，ELISA，bleed，WB,proteinA purify	90 days
QY-1017	cDNA → anti-sera	expression plasmid construct,protein  express and purify，eimmunize，ELISA ，bleed，WB	100 days
QY-1018	cDNA → antibody （protinA purification）	expression plasmid construct,protein  express and purify，eimmunize，ELISA ，bleed，WB,ProteinA，	110 days
QY-1019	DNA sequence  → anti-sera	gene synthesis,expression plasmid construct,protein express and purify ，eimmunize，ELISA，bleed，WB	120 days
QY-1020	DNA sequence → antibody （protinA purification）	gene synthesis,expression plasmid construct,protein express and purify，eimmunize，ELISA，bleed，WB,  proteinA purify	130 days
QY-1021	protein → anti-sera （goat）	antigen QC，immunize goat，ELISA ，bleed	70 days
QY-1022	protein → anti-sera （chicken）	antigen QC，immunize chicken，ELISA ，bleed	70 days

 
 
Custom Monoclonal Antibody Services
Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same because they are made by identical immune cells that are all clones of a unique parent cell. The mAb has many advantages: specificity, homogeneity, high efficiency and infinity. The use of monoclonal antibodies is numerous and includes the prevention, diagnosis, and treatment of disease.
Monoclonal antibodies have a single, selected specificity and which, usually, are secreted continuously by “immortalized” hybridoma cells. A hybridoma is a biologically constructed hybrid of an antibody- producing lymphoid cell and a malignant (immortal) myeloma cell. Hybridoma technology is a technology of forming hybrid cell lines (called hybridomas) by fusing a specific antibody-producing B cell with a myeloma (B cell cancer) cell that is selected for its ability to grow in tissue culture and for an absence of antibody chain synthesis. The antibodies produced by the hybridoma are all of a single specificity and are therefore monoclonal antibodies (in contrast to polyclonal antibodies).

Service Code	Service	Brief Description	Time
QY-2001	protein → monoclonal antibody	1.2-5 hybridoma cell lines; 2.2-5 mg protein A purified mAb per cell line. 3.mAb ELISA titer ≥80,000	18-24 weeks
QY-2002	peptide → monoclonal antibody	1.2-5 hybridoma cell lines; 2.2-5 mg protein A purified mAb per cell line. 3.mAb ELISA titer ≥80,000	20-26 weeks
QY-2003	phosphor-peptide → monoclonal antibody	1.2-5 hybridoma cell lines; 2.2-5 mg protein A purified mAb per cell line; 3.mAb specific to phosphor-peptide: ELISA titer ≥80,000; 4.mAb titer to phosphor-peptide/non-phosphor-peptide ≥16 (ELISA and Dot Blot)	20-26 weeks
QY-2004	small molecules compound → monoclonal antibody	1.2-5 hybridoma cell lines; 2.2-5 mg protein A purified mAb per cell line; 3.mAb to conjugated compond: ELISA titer ≥80,000; 4.mAb's interaction with conjugated compond can be competitived with small molecules compound	20-26 weeks
QY-2005	protein → monoclonal antibody （pairs,used in Double sandwich ELISA)	1.2-5 hybridoma cell lines; 2.a pair of antibodies,5 mg coated antibody, 5mg detected antibody	22-30 weeks

 
 
 
 
 
 

Custom phosphorylation antibody services

Custom phosphorylation antibody services The custom phosphorylation antibody services provided by PhtdPeptides Co., Ltd. include peptide antigen design, coupling, immune, antigen affinity purification. The project starts after confirmed by the client, and the final delivery antibody will be checked through phosphorylated peptides and non phosphorylated peptide double testing. Phosphorylation antibody can specifically identify the phosphorylated amino acid sites. Phosphorylation antibody can be used to distinguish between the existence of protein phosphorylation and non phosphorylated form, can be used for qualitative and quantitative analysis of protein phosphorylation. PhtdPeptides has a wealth of experience in analysis, design and synthesis of phosphorylated peptide and phosphorylation antibody preparation technology. We provide phosphorylation polyclonal antibody and phosphorylation of monoclonal antibodies services. In the project, animal immune serum collected is purified through phosphorylated peptides affinity chromatography column firstly, then the preliminary purification antibody is absorbed with non phosphorylated peptide affinity chromatography column, so the final delivery phosphorylation antibody is confirmed with the BSA crosslinking of phosphorylated peptide and non phosphorylated peptide double test to make sure the phosphorylation antibody delivery quality. Phosphorylation polyclonal antibody preparation (rabbit)  Firstly, Design and synthesis of phosphorylated peptide, synthesis 10 mg of phosphorylated peptides and 5 mg of phosphorylated peptides. Secondly, Peptide coupling, synthesis peptides coupling with carrier protein (KLH, BSA), 2 ~ 3 mg peptide (non phosphorylated peptide and phosphorylated peptides) will be provided. Thirdly, Immune rabbit, serum before the immune, immune four times and serum. 1~ 2 ml serumbefore the immune will be provided. Fourthly, Small-scale purified, serum sample collection and detection. 2 ~ 5 mg phosphorylation antibody and testing result by ELISA will be provided. Fifthly, Antigen affinity purification, preparation phosphorylation affinity purification column, non phosphorylated peptides affinity column to remove cross reaction. Project report will be provided. Sixthly, Detect titer by ELISA.  Phosphorylation of monoclonal antibody preparation (mouse)  Firstly, Design and synthesis of phosphorylated peptide, synthesis 10 mg of phosphorylated peptides and 5 mg of phosphorylated peptides. Secondly, Peptide coupling, synthesis peptides coupling with carrier protein (KLH, BSA), 2 ~ 3 mg peptide (non phosphorylated peptide and phosphorylated peptides) will be provided. Thirdly, Animal immunization and detection, immune mice and tested by ELISA with phosphorylation and non phosphorylated peptide. 5 mg modified peptides, 3 mg undecorated peptides, antibodies and 50 ul serum before immune will be provided. Fourthly, Cell fusion, phosphorylated peptide showed the best immune response, mice spleen cells and myeloma cells for cell fusion. 2-5 strains hybridoma, 3-8 mg purified antibody (2 strains) and ELISA test results will be provided. Fifthly, Proliferation and selection, detect the positive cloning by ELISA and the subcloning. Subtype identification results and complete project report will be provided. Sixthly, Ascites preparation and purification, purification of Protein G. Seventhly, Detect titer by ELISA.

 
Antibody Purification，Label，Immunoassay Services
An immunoassay is a biochemical test that measures the presence or concentration of a substance in solutions that frequently contain a complex mixture of substances. Such assays are based on the unique ability of an antibody to bind with high specificity to one or a very limited group of molecules. A molecule that binds to an antibody is called an antigen. Immunoassays can be carried out for either member of an antigen/antibody pair. The high specific and effective antibody is a key to a successful immunoassay.Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower background by removing any non-specific proteins. Antibody labeling can amplify signal and increase sensitivity. There are many immunoassay techniques such as ELISA、WB、IP and IHC etc.
Phtdpeptides has a leading antibody services technology, advanced equipments and professional technician, provides many services including antibody purification, antibody labeling and immunoassay techniques.
 

Service Code	Service	Brief Description	Time
QY-3001	Antibody Purification （ammonium sulfate)	1.ammonium sulfate precipitation of antisera（≤50 ml) 2.Detect concentration by UV absorption 3.Elisa	1 week
QY-3002	Antibody Purification （ProteinA/G）	1.antisera（≤50 ml) purification by proteinA/g 2.detect concentration by UV absorption 3.Elisa	1 week
QY-3003	Antibody Purification （antigen affinity）	1.antigen QC 2.antigen affinity resin prepare 3.antigen affinity purification（≤50 ml) 4.Detect concentration by UV absorption 5.Elisa	2 week
QY-3004	Antibody Label（HRP）	1.antibody QC 2.antibody label with HRP 3.ELSIA	1 week
QY-3005	Antibody Label （Biotin）	1.antibody QC 2.antibody label with Biotin 3.label ratio test	1 week
QY-3006	Antibody Label（FITC）	1.antibody QC 2.antibody label with FITC 3.label ratio test	1 week
QY-3007	ELISA	Ag or Ab detect in indirect method,sandwich,or competitive methods	1-2 weeks
QY-3008	Western Blot	Totol protein extract,SDS-PAGE,transfer,hybrid,detect&analysis	2-3 weeks
QY-3009	Immunoprecipitation	Preparation of lysates，Pre-clearing the lysates,Immunoprecipitation	2-3 weeks
QY-3010	Immunohistochemistry （IHC）	Deparaffinization/Rehydration,Antigen Unmasking,Staining	2-3 weeks

 
 

Prokaryotic proteins purification services

Prokaryotic proteins purification services
prokaryotic expression is the method of inserting cloned gene into suitable vector and introducing into E. coli for expression of large amounts of protein.prokaryotic expression system is by far the most mature and reliable protein expression system which rapidly express and purify a variety of protein sources. Prepared recombinant protein can be used for drug screening, structural biology, cell biology, proteomics and other fields of biomedical.Based on different expression requirements, such as volume of expression, viability of targeted protein and different purification method, we choose proper expression system and corresponding expression strategy. We make use of our mature and steady protein purification system to optimize expression terms and purify targeted protein.
Service Number: QY-4003 
Service Items: 
Select of plasmid transformation and high expression strain
Cultivate Transformed bacteria flask and Inducible expression
Purify of Targeted proteins
Detect expression and purification through SDS-PAGE 
Final material delivery:
Initial culture conditions and reasonable purification process; purified protein (1mg, more than 85% purity)
SDS-PAGE protein electrophoresis results and detailed experimental records
Product Report (including relevant data, SDS-PAGE and plasmid sequencing results, etc.)
Sample requirement:
Provide cloned prokaryotic expression vector or plasmid or strain in cloning vector
Provide relevant information and Nucleic acid/protein sequence,use bioinformatics methods to analyze and make concrete and feasible program of the experiment
Attention:
1.Expression vector provided by client shall be transformed and expressed, proving with the expression of the target protein, plasmid ≥1ug
2.Expression protein should be equipped with His and GST label.
 
Antibody Purification，Label，Immunoassay Services
An immunoassay is a biochemical test that measures the presence or concentration of a substance in solutions that frequently contain a complex mixture of substances. Such assays are based on the unique ability of an antibody to bind with high specificity to one or a very limited group of molecules. A molecule that binds to an antibody is called an antigen. Immunoassays can be carried out for either member of an antigen/antibody pair. The high specific and effective antibody is a key to a successful immunoassay.Antibody purification is performed to concentrate and enrich antigen-specific antibodies and lower background by removing any non-specific proteins. Antibody labeling can amplify signal and increase sensitivity. There are many immunoassay techniques such as ELISA、WB、IP and IHC etc.
Phtdpeptides has a leading antibody services technology, advanced equipments and professional technician, provides many services including antibody purification, antibody labeling and immunoassay techniques.
 

Service Code	Service	Brief Description	Time
QY-3001	Antibody Purification （ammonium sulfate)	1.ammonium sulfate precipitation of antisera（≤50 ml) 2.Detect concentration by UV absorption 3.Elisa	1 week
QY-3002	Antibody Purification （ProteinA/G）	1.antisera（≤50 ml) purification by proteinA/g 2.detect concentration by UV absorption 3.Elisa	1 week
QY-3003	Antibody Purification （antigen affinity）	1.antigen QC 2.antigen affinity resin prepare 3.antigen affinity purification（≤50 ml) 4.Detect concentration by UV absorption 5.Elisa	2 week
QY-3004	Antibody Label（HRP）	1.antibody QC 2.antibody label with HRP 3.ELSIA	1 week
QY-3005	Antibody Label （Biotin）	1.antibody QC 2.antibody label with Biotin 3.label ratio test	1 week
QY-3006	Antibody Label（FITC）	1.antibody QC 2.antibody label with FITC 3.label ratio test	1 week
QY-3007	ELISA	Ag or Ab detect in indirect method,sandwich,or competitive methods	1-2 weeks
QY-3008	Western Blot	Totol protein extract,SDS-PAGE,transfer,hybrid,detect&analysis	2-3 weeks
QY-3009	Immunoprecipitation	Preparation of lysates，Pre-clearing the lysates,Immunoprecipitation	2-3 weeks
QY-3010	Immunohistochemistry （IHC）	Deparaffinization/Rehydration,Antigen Unmasking,Staining	2-3 weeks